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KMID : 0604520070330020061
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Journal of the Society of Cosmetic Scientists of Korea
2007 Volume.33 No. 2 p.61 ~ p.67
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Evaluation of Antioxidant Potential of Extract/Fractions of Equisetum arvense (I)
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Yang Hee-Jung
Park Soo-Nam
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Abstract
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In this study, the antioxidative effects of Equisetum arvense extracts were investigated. The free radical (1,1 diphenyl-2-picrylhydrazyl, DPPH) scavenging activity () of extract/fractions of Equisetum arvense was in the order: 50 % ethanol extract () < ethylacetate fraction () < deglycosylated flavonoid aglycone fraction (). Reactive oxygen species (ROS) scavenging activities () of some Equisetum arvense extracts on ROS generated in system were investigated using the luminol- dependent chemiluminescence assay. The order of ROS scavenging activity was deglycosylated flavonoid aglycone fraction (, ) < 50 % ethanol extract () < ethylacetate fraction (). Ethylacetate fraction showed the most prominent scavenging activity. The protective effects of extract/fractions of Equisetum arvense on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethanol extract (50%) suppressed photohemolysis in a concentration dependent manner, particularly deglycosylated aglycone extract exhibited the most prominent celluar protective effect (, 161.10 min at ). These results indicate that extract/fractions of Equisetum arvense can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging and other ROS, and protect cellular membranes against ROS.
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KEYWORD
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Equisetum arvense, free radical, reactive oxygen species, antioxidative activity, photohemolysis
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